All experimental protocols were approved by the Ohio State University Institutional Animal Care and Use Committee (protocol# 2016A00000068) on July 14, 2016. These findings may be useful for refining hypotheses regarding the contributions of these cell types to regulating hippocampal function and their potential therapeutic uses. Notably, neural stem/progenitor cells were present at a similar density as several cell types known to have potent functional roles via their secretome. We did not observe any significant sex differences in density of any cell population. Of the cell types quantified, we found that GFAP +SOX2 + stellate astrocytes were the most numerous, followed by CD31 + endothelia, GFAP –SOX2 + intermediate progenitors, Olig2 + oligodendrocytes, Iba1 + microglia, and GFAP +SOX2 + radial glia-like neural stem cells. Here, we apply immunohistochemical methods to estimate cell density of neural stem/progenitor cells versus other major classes of glial and endothelial cell types that are potentially secretory in the dentate gyrus of adult mice. In contrast, the non-neurogenic functions of adult neural stem cells and their immediate progeny, such as secretion of bioactive growth factors and expression of extracellular matrix-modifying proteins, lack similar framing due to few estimates of their number versus other prominent secretory cells. Hypotheses regarding the role of adult-born neuron synaptic integration in hippocampal circuit function are framed by robust estimations of adult-born versus pre/perinatally-born neuron number.
The dentate gyrus subregion of the mammalian hippocampus is an adult neural stem cell niche and site of lifelong neurogenesis. Source of Support: The study was partially supported by a R00 Pathway to Independence Award from NIH/NINDS (R00NS089938 to EDK), Conflict of Interest: None Joshua D Rieskamp 1, Patricia Sarchet 2, Bryon M Smith 2, Elizabeth D Kirby PhD 3ġ Neuroscience Graduate Program Department of Psychology, The Ohio State University, Columbus, OH, USA 2 Department of Psychology, The Ohio State University, Columbus, OH, USA 3 Department of Psychology Department of Neuroscience Chronic Brain Injury Program, The Ohio State University, Columbus, OH, USA Date of SubmissionĬorrespondence Address: Elizabeth D Kirbyĭepartment of Psychology Department of Neuroscience Chronic Brain Injury Program, The Ohio State University, Columbus, OH Estimation of the density of neural, glial, and endothelial lineage cells in the adult mouse dentate gyrus
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